Skip to main content
. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Chem Biol. 2021 Jan 25;17(4):387–393. doi: 10.1038/s41589-020-00721-2

Extended Data Fig. 5. Comparison of the REC1 domain in Cas12g and Cas12b, and crRNA guide mismatch assay for Cas12g.

Extended Data Fig. 5

a, The tracrRNA scaffolding duplex 1 is bound to the concave surface of the REC1 domain in the Cas12g-sgRNA binary complex. b, The crRNA-target DNA duplex is bound to the concave surface of the REC1 domain in the Cas12b-sgRNA-DNA complex. c, The tracrRNA scaffolding duplex 1 is bound to the concave surface of the REC1 domain in the Cas12g-sgRNA-target RNA complex. d, RNA cleavage assay using wild-type and mutant target RNAs. The results shown are representative of three experiments.