Skip to main content
. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Chem Biol. 2021 Jan 25;17(4):387–393. doi: 10.1038/s41589-020-00721-2

Extended Data Fig. 6. Cryo-EM data processing for the Cas12g-sgRNA-target RNA complex.

Extended Data Fig. 6

a, A representative raw cryo-EM micrograph of the Cas12g-sgRNA-target RNA complex from a total of 987 micrographs. b, Representative 2D class averages from a total of 100 images. c, 3D classification. Three major classes were observed. Classes II and III did not result in good reconstructions and likely represent damaged particles. d, 3D refinement for Class I. Angular distribution is shown on the right. e, Plot of the global half-map FSC (solid red line), map-to-model FSC (solid orange line), and spread of directional resolution values (±1σ from mean, green dotted lines; the blue bars indicate a histogram of 100 such values evenly sampled over the 3D FSC). FSC plot for the reconstruction suggests an average resolution of 4.8 Å. f, Local resolution map in two views.