Skip to main content
. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Chem Biol. 2021 Jan 25;17(4):387–393. doi: 10.1038/s41589-020-00721-2

Extended Data Fig. 8. Binding assay between the Cas12g-sgRNA complex and target nucleic acids.

Extended Data Fig. 8

Upper: SEC profiles for the Cas12g-sgRNA complex incubated with different targets including target RNA, target RNA with mismatches at positions 16 and 17, target RNA with mismatches at positions 18 and 19, and target ssDNA. UV absorbance curves at 280 nm are shown. Lower: Urea-PAGE (15%) analysis of the elution fractions from SEC as indicated. The results shown are representative of three experiments.