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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Chem Biol. 2021 Jan 25;17(4):387–393. doi: 10.1038/s41589-020-00721-2

Extended Data Fig. 4. Collateral cleavage and stoichiometric titration assays for Cas12g.

Extended Data Fig. 4.

a, Collateral cleavage of unrelated ssDNA by Cas12g. The results shown are representative of three experiments. b, Collateral cleavage of unrelated ssRNA by Cas12g. The results shown are representative of three experiments. c, Stoichiometric titration assay indicates that Cas12g is a multiple turnover enzyme towards RNA substrate. Each point represents the cleavage ratio after 30 minutes at 37°C. Error bars represent mean ± SD, where n = 3 replicates.