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. Author manuscript; available in PMC: 2022 Jun 29.
Published in final edited form as: Biochemistry. 2021 Jun 14;60(25):1971–1982. doi: 10.1021/acs.biochem.1c00270

Figure 2: TBIA mechanism and characterization.

Figure 2:

(A) Reaction of TBIA with RNA yields both crosslinks and mono-adducts. IA strictly forms mono-adducts. (B) Reaction of the RNase P RNA with no reagent (–), IA (mono-adduct), or TBIA (crosslinking reagent), visualized by denaturing electrophoresis. (C) Hydrolysis of TBIA in buffer. Reaction was monitored by UV absorbance at 296 nm; absorbance as a function of time was fit to a double exponential rate equation.