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. 2021 Jun 21;11:612952. doi: 10.3389/fonc.2021.612952

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Copyright © 2021 Ye, Zheng, Zeng, Wei, Huang, Wang, Liu, Lin, Chen, Zheng and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The verification of identified genes using PCR and HPA database. (A) Q-PCR was used to validate the expressions of 13 metabolism-related genes (GSTA2, POLD3, GLA, GGT5, DCK, CKMT2, ASAH1, OPLAH, ME1, ACYP1, NNMT, POLR1A, and RDH12) between MKN-45 and GES-1. (B) Q-PCR was used to validate the expressions of 13 metabolism-related genes (GSTA2, POLD3, GLA, GGT5, DCK, CKMT2, ASAH1, OPLAH, ME1, ACYP1, NNMT, POLR1A, and RDH12) between AGS and GES-1. (C) Protein expression level of GSTA2 was verified in the Human Protein Atlas (HPA). (D) Protein expression level of ASAH1 was verified in the HPA. *p < 0.05, **p < 0.01, and ***p < 0.001. NS, None significance.