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. Author manuscript; available in PMC: 2021 Jul 5.
Published in final edited form as: J Vis Exp. 2020 May 15;(159):10.3791/61446. doi: 10.3791/61446

Figure 1: E14 ESCs in culture.

Figure 1:

(A) The light microscope images show E14 cells (black arrow) growing in colonies atop the MEFs. E14 colonies continue to proliferate as seen in the colony size difference between day 1 and day 3 cultures. (B) Confirmation of the pluripotency of E14 ESCs by the alkaline phosphatase (AP) stain.