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. Author manuscript; available in PMC: 2022 Apr 2.
Published in final edited form as: J Proteome Res. 2021 Mar 17;20(4):1951–1965. doi: 10.1021/acs.jproteome.0c00964

Table 2.

Summary of factors and effect sizes by NCE

Property Explained Variance of CID vs HCD
Y+1H Y+2H
NCE 20 23 25 28 30 35 20 23 25 28 30 35
Mass / size 12% 15% 18% 24% 24% 31% 1% 1% 1% 3% 4% 13%
Basic residue content 6% 8% 9% 11% 11% 11% 3% 3% 4% 5% 5% 7%
Bond cleavage <1% 1% 1% 1% 1% 1% <1% 1% 1% 1% 1% 2%