KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| TruStain fcX (anti-mouse CD16/32) antibody | Biolegend | RRID:AB_1574975 |
| Biotin anti-mouse TER-119/Erythroid Cells antibody | Biolegend | RRID:AB_313705 |
| Biotin anti-mouse CD3ε antibody | Biolegend | RRID:AB_312669 |
| Biotin anti-mouse/human CD45R/B220 Antibody | Biolegend | RRID:AB_312989 |
| Biotin anti-mouse NK-1.1 antibody | Biolegend | RRID:AB_313391 |
| Biotin anti-mouse CD34 antibody | Biolegend | RRID:AB_1236371 |
| Biotin anti-mouse CD117 (c-kit) antibody | Biolegend | RRID:AB_313213 |
| PE anti-mouse CD14 antibody | Biolegend | RRID:AB_2728189 |
| PE/Dazzle 594 anti-mouse CD115 (CSF-1R) antibody | Biolegend | RRID:AB_2566522 |
| PE/Cy7 anti-mouse F4/80 antibody | Biolegend | RRID:AB_893478 |
| PE anti-mouse CD192 (CCR2) antibody | Biolegend | RRID:AB_2616982 |
| APC anti-mouse CD192 (CCR2) antibody | Biolegend | RRID:AB_2810415 |
| Brilliant Violet 421 anti-mouse/human CD11b antibody | Biolegend | RRID:AB_10897942 |
| Brilliant Violet 510 anti-mouse Ly6C antibody | Biolegend | RRID:AB_2562351 |
| Brilliant Violet 605 anti-mouse CD64 (FcgammaRI) antibody | Biolegend | RRID:AB_2629778 |
| Brilliant Violet 650 anti-mouse Ly6G antibody | Biolegend | RRID:AB_2565881 |
| Brilliant Violet 785 anti-mouse CX3CR1 antibody | Biolegend | RRID:AB_2565938 |
| APC anti-mouse CD62L antibody | Biolegend | RRID:AB_313098 |
| FITC anti-mouse CD86 antibody | Biolegend | RRID:AB_313148 |
| APC R700 anti-mouse CD45 | BD BioSciences | RRID:AB_2739257 |
| BUV395 anti-mouse I-A/I-E | BD BioSciences | RRID:AB_2741827 |
| BUV737 anti-mouse CD11c | BD BioSciences | RRID:AB_2873433 |
| Monoclonal rabbit anti-c-Myc antibody | Abcam | RRID:AB_731658 |
| Monoclonal rabbit anti-Hif1α antibody | Abcam | RRID:AB_2732807 |
| Polyclonal rabbit anti-Laminb1 antibody | Abcam | RRID:AB_443298 |
| Polyclonal rabbit anti-LDHA antibody | Cell Signaling | RRID:AB_2137173 |
| Polyclonal rabbit anti-phosphorylated JNK antibody | Cell Signaling | RRID:AB_331659 |
| Polyclonal rabbit anti-phosphorylated ERK1/2 antibody | Cell Signaling | RRID:AB_331646 |
| Monoclonal rabbit anti-phosphorylated p38 antibody | Cell Signaling | RRID:AB_331762 |
| Polyclonal rabbit anti-p38 antibody | Cell Signaling | RRID:AB_330713 |
| Polyclonal rabbit anti-IκBα antibody | Cell Signaling | RRID:AB_331623 |
| Monoclonal rabbit anti-IRF4 antibody | Cell Signaling | RRID:AB_2798709 |
| Monoclonal rabbit anti-phosphorylated Akt antibody | Cell Signaling | RRID:AB_2315049 |
| Polyclonal rabbit anti-A20 antibody | Cell Signaling | RRID:AB_2204524 |
| Monoclonal rabbit anti-ATP-Citrate Lyase antibody | Cell Signaling | RRID:AB_2798203 |
| Polyclonal rabbit anti-phosphorylated ATP-Citrate Lyase antibody | Cell Signaling | RRID:AB_2257987 |
| Monoclonal rabbit anti-phosphorylated p70 S6 Kinase antibody | Cell Signaling | RRID:AB_2269803 |
| Monoclonal rabbit anti-p70 S6 Kinase | Cell Signaling | RRID:AB_390722 |
| Polyclonal rabbit anti-phosphorylated 4E-BP1 antibody | Cell Signaling | RRID:AB_330947 |
| Monoclonal rabbit anti-4E-BP1 antibody | Cell Signaling | RRID:AB_2097841 |
| Monoclonal rabbit anti-p65 antibody | Cell Signaling | RRID:AB_823578 |
| Monoclonal mouse anti-α-tubulin antibody | Sigma Aldrich | RRID:AB_477593 |
| Polyclonal anti-mouse IL-1 beta/IL-1F2 Biotinylated antibody | R&D systems | RRID:AB_356450 |
| Bacterial and virus strains | ||
| Listeria monocytogenes (wild-type strain 10403S) | Serbina et al., 2003 | N/A |
| Biological samples | ||
| Human Peripheral Blood | New York Blood Center | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Ultrapure LPS-EB (LPS from E. coli O111:B4) | InvivoGen | Cat#tlrl-3pelps |
| LPS from Escherichia coli strain O55:B5 | Sigma Aldrich | Cat#L2880 |
| Mouse recombinant IL-4 | Miltenyi Biotec | Cat#130-097-757 |
| Pam3Cys | EMC Microcollections | Cat#L2000 |
| Human recombinant M-CSF | Peprotech | Cat#300-25 |
| L-(+)-Lactic acid | Sigma Aldrich | Cat#L6402 |
| 2-Deoxy-D-glucose (2-DG) | Sigma Aldrich | Cat#D8375 |
| UK-5099 | Sigma Aldrich | Cat#PZ0160 |
| Etomoxir | Sigma Aldrich | Cat#E1905 |
| Pefabloc SC | Sigma Aldrich | Cat#76307 |
| Deoxyribonuclease I | Sigma Aldrich | Cat#DN25 |
| Collagenase, Type IV | Thermo Fisher Scientific | Cat#17104019 |
| CHC | Tocris Bioscience | Cat#5029 |
| FX11 | EMD Millipore | Cat#427218 |
| Polybrene | Santa Cruz | Cat#sc-134220 |
| Gentamycin | Thermo Fisher Scientific | Cat#15710064 |
| FuGENE(R) HD Transfection Reagent | Promega | Cat#E2312 |
| TransIT-TKO® Transfection Reagent | Mirus Bio | Cat#MIR 2150 |
| SYTO 24 green fluorescent nucleic acid stain | Molecular Probes | Cat#S7559 |
| Brain Heart Infusion | BD BioSciences | Cat#BD 237500 |
| Brilliant Stain Buffer | BD BioSciences | Cat#563794 |
| PerCP/Cy5.5 Streptavidin | Biolegend | Cat#405214 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | Thermo Fisher Scientific | Cat#D1306 |
| eBioscience Fixable Viability Dye eFluor 455UV | Thermo Fisher Scientific | Cat#65-0868-18 |
| CountBright Absolute Counting Beads, for flow cytometry | Thermo Fisher Scientific | Cat#C36950 |
| UltraComp eBeads Compensation Beads | Thermo Fisher Scientific | Cat#01-2222-42 |
| Streptavidin-HRP | R&D systems | Cat#DY998 |
| Critical commercial assays | ||
| First strand cDNA synthesis kit | Fermentas | Cat#K1622 |
| RNeasy mini kit | QIAGEN | Cat#74104 |
| Fast SYBR Green Master Mix | Life Technologies | Cat#4385618 |
| Mouse Magnetic Luminex Assay | R&D systems | Custom made |
| Lactate Dehydrogenase Assay Kit | Abcam | Cat#ab102526 |
| Seahorse XF Glycolysis Stress Test Kit | Agilent | Cat#103020-100 |
| Seahorse XF Mito Stress Test Kit | Agilent | Cat#103015-100 |
| Cell Counting Kit-8 | Dojindo Laboratories | Cat#CK04-01 |
| Experimental models: organisms/strains | ||
| LysM Cre: B6.129P2-Lyz2tm1(cre)Ifo/J | Jackson Laboratory | RRID:IMSR_JAX:004781 |
| Myc flox: B6.129S6-Myctm2Fwa/Mmjax | Jackson Laboratory | RRID:MMRRC_032046-JAX |
| Hif1a flox: B6.129-Hif1atm3Rsjo/J | Jackson Laboratory | RRID:IMSR_JAX:007561 |
| C57BL/6J | Jackson Laboratory | RRID:IMSR_JAX:000664 |
| IRF4 KO | A gift from Dr. Kelvin P. Lee; Utley et al., 2020 | N/A |
| Oligonucleotides | ||
| See Table S2 | ||
| Recombinant DNA | ||
| pMXs-c-Myc | Takahashi and Yamanaka, 2006 | RRID:Addgene_13375 |
| pMXs-gw | Takahashi and Yamanaka, 2006 | RRID:Addgene_18656 |
| Deposited data | ||
| RNA-seq | This paper | GEO: GSE164475 |
| Software and algorithms | ||
| Bowtie2 version 2.2.6 | Langmead and Salzberg, 2012 | https://www.bioinformatics.babraham.ac.uk/projects/fastqc |
| STAR Aligner | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| edgeR | Robinson et al., 2010 | https://www.bioconductor.org/packages/release/bioc/html/edgeR.html |
| R | https://www.R-project.org/ | |
| Cuffdiff2 | Trapnell et al., 2013 | http://cole-trapnell-lab.github.io/cufflinks/cuffdiff/ |
| Gene Set Enrichment Analysis (GSEA) | Broad Institute, Inc. | https://www.gsea-msigdb.org/gsea/msigdb/index.jsp |
| MassHunter Quantitative Analysis (v. B.09.00) | Agilent Technologies | https://www.agilent.com/en/product/software-informatics/mass-spectrometry-software/data-analysis/quantitative-analysis |
| Mass Profiler Professional (v. 15.0) | Agilent Technologies | https://www.agilent.com/en/product/software-informatics/mass-spectrometry-software/data-analysis/mass-profiler-professional-software |
| Metaboanalyst | Chong et al., 2019 | https://www.metaboanalyst.ca |
| Seahorse Wave Desktop Software | Agilent Technologies | https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-software/seahorse-wave-desktop-software-740897 |
| Flowjo (v10.7.1) | BD Biosciences | https://www.flowjo.com/solutions/flowjo/downloads/ |
| GraphPad Prism 8 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |