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. 2021 Jul 6;345:130411. doi: 10.1016/j.snb.2021.130411

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Fig. 4 — Optimization of RT-LAMP amplification. Real-time RT-LAMP amplification curves with different concentrations of SARS-CoV-2 N gene before optimization (A) and after optimization (B). (C) Time to positive (TTP) for RT-LAMP amplification before (left) and after (right) optimization (lower value and lighter colors indicate faster amplification). TTP is defined as the time when the fluorescent intensity passes 3000. (D) The time needed for the CLAP assay with different concentrations of SARS-CoV-2. (E) Visual detection of SARS-CoV-2 by CLAP after optimization. NTC, no-template control, Flu, Flu-HA gene.