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. 2021 Jun 25;2021:5040326. doi: 10.1155/2021/5040326

Figure 3.

Figure 3

Inhibition of autophagy reduced Rg5-induced caspase-3 activity and cell apoptosis. 5 mM 3-MA combining or alone with 160 nM Rg5 was used to treat MG-63 cells for 12 h and for caspase-3 and LC3 autophagy-related protein detection by western blotting or FACS. (a) Representative image of proteins detected by western blotting. (b) The ratio of LC3-II/LC3-I in MG-63 cells. (c) The ratio of cleaved caspase-3/caspase-3 in MG-63 cells. (d) The MFI of LC3 in MG-63 cells detected by FACS. (e, f) MG-63 cell apoptosis detected by FACS. The data are shown as M ± SD (n = 3, nsp > 0.05, #p < 0.05, 3-MA treatment comparing with control; ∗p < 0.05, ∗∗p < 0.01 vs. control).