Skip to main content
. 2021 Jun 28;2021:9960241. doi: 10.1155/2021/9960241

Figure 5.

Figure 5

miR-450a-2-3p decreases TGF-β-induced activation and proliferation of CFs. miR-450a-2-3p, α-SMA, COL1, and COL3 mRNA expression in CFs transfected with mimics (a)–(d) and inhibitor (i)–(l) was detected using RT-qPCR. The protein levels of α-SMA, COL1, and COL3 in CFs transfected with mimics (e)–(h) and inhibitor (m)–(p) were determined using western blotting. The expression level of the miRNAs analyzed was normalized to the expression level of U6, and the expression level of mRNAs and proteins was normalized to the expression level of β-actin. Representative images of immunofluorescence staining for α-SMA in CFs transfected with mimics and inhibitor (r) (200× magnification) showed the antifibrotic effect of miR-450a-2-3p. CF proliferation measured by the CCK-8 assay after mimics (s) and inhibitor (t) treatment. Data is presented as the mean ± SEM of three independent experiments. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.