Figure 7.

miR-450a-2-3p targets ERK1/2 (MAPK1). Protein (a) and mRNA (b) levels of ERK1/2 in CFs and HUVECS transfected with mimics or inhibitor were measured using western blot or RT-qPCR, respectively. The expression levels of mRNA and proteins were normalized to that of actin. (c) Relative luciferase activity of WT or mutant MAPK1 3′-UTR reporter cotransfected with miR-450a-2-3p mimics. Data is presented as the mean ± SEM of three independent experiments. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.0001.