Fig. 1. Loss of Bcor induces hematopoietic changes in mice.
A RBCs count (i) mean corpuscolar volume (MCV) (ii) and platelets count (iii) in PB of Bcor−/−, Bcor+/− and WT from 12 months old mice. B (i) Megakaryocyte-erythroid progenitors (MEP Lin − /Sca1-/Kit+CD34-FCgRII/IIIlo/-) in BM of Bcor−/− and WT 6 months old mice. (ii) Representative flow-cytometric analysis of immature megakaryocytic compartments, megakaryocyte progenitor (MkP) and total number of MkP (iii) in BM of Bcor−/−, Bcor+/− and WT mice. (iv) total number of CD41 + cells in BM of Bcor−/−, Bcor+/− and WT 6 months old mice. (iv) Apoptosis in CD41 cells in WT and Bcor−/− 3 months old mice. 1 × 106 cells from BM were plated for 6 h in RPMI + BRDU, than counted and stained. C (i) Kaplan–Mayer plot of mouse survival according to the indicated genotypes (n = 44 WT,19 Bcor+/−, 37 Bcor−/−). (ii) Pie charts showing the different causes of mortality: 56% of deaths occurred in the Bcor mutant cohort in the presence of well-defined hematological abnormalities. In the remaining mice, pathological examinations were consistent with the occurrence of hepatic carcinoma (8%), intestinal/lung tumors (4%), possibly due to Cre-recombinase leakiness. Notably, 32% of deaths were of unknown origin. *p < 0.05, **p < 0.01; ***p < 0.001 unpaired t-test with Welch’s correction.