Fig. 5. Compound Bcor and Dnmt3a loss induce a strong displacement toward the erythroid profile in mice.

A Representative flow-cytometric analysis (i) of stem cell compartment, long term hematopoietic stem cells (LT-HSC Lin⁻/Sca1⁺/Kit⁺CD34l^o/−FLT3^-), short term hematopoietic stem cells (ST-HSC Lin⁻/Sca1⁺/Kit⁺CD34⁺FLT3^-) and multipotent progenitors (MPP Lin⁻/Sca1⁺/Kit⁺CD34⁺FLT3⁺) in BM of Bcor^−/−Dnmt3a^−/−, Bcor^−/−, Dnmt3a^−/−and WT mice. Total number (Right panel) of LT-HSC (ii), ST-HSC (iii) and MPP (iv) in BM of Bcor^−/−Dnmt3a^−/−, Bcor^−/−, Dnmt3a^−/−and WT mice. B Representative flow-cytometric analysis (i) of progenitors cell compartment, granulocyte-macrophage progenitors (GMP Lin⁻/Sca1⁻/Kit⁺CD34⁺FCgRII/III⁺), common-myeloid (CMP Lin⁻/Sca1⁻/Kit⁺CD34⁺FCgRII/III⁻) and megakaryocyte-erythroid progenitors (MEP Lin⁻/Sca1⁻/Kit⁺CD34⁻FCgRII/III^lo/−) in BM of Bcor^−/−Dnmt3a^−/−, Bcor^−/−, Dnmt3a^−/−and WT mice. Total number of GMP (ii), CMP (iii) and MEP (iv) in BM of Bcor^−/−Dnmt3a^−/−, Bcor^−/−, Dnmt3a^−/− and WT mice. (n = 16, 12, 10, 9) (v) Total number of (Pre-MegE Lin⁻/FCgRII/III⁻ CD150⁺CD105⁻) and Pre-CFUe (Lin⁻/FCgRII/III^- CD150⁺CD105⁺) in BM of Bcor^−/−Dnmt3a^−/−, Bcor^−/−, Dnmt3a^−/− and WT mice. (n = 16, 12, 10, 9) C Summary of BM hemopoietic development in mice; red arrows indicate the deregulated populations. *p < 0.05, **p < 0.01; ***p < 0.001 unpaired t-test with Welch’s correction. .