Fig. 6. miR-10a regulates the switch between p53-mediated autophagy and apoptosis in AML by repressing autophagy induction.

Cells in which miR-10a was inhibited were significantly less likely to undergo autophagy as measured by MDC fluorescence (a) and LC3-II protein accumulation (b) following treatment with Nutlin-3a or cytarabine. c Scrambled miRNA inhibitor-transfected control cells undergo cell cycle arrest in the G₀/G₁ and S phases in response to cytarabine and Nutlin-3a treatment, respectively. When miR-10a was inhibited however, cell cycle arrest was attenuated, with significantly increased numbers of cells progressing through G₀/G₁ and S checkpoints. (*p < 0.05 by Student’s T test with error bars representing SD (3-6 replicates per experiment)).