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. 2021 Mar 21;58(7):3515–3528. doi: 10.1007/s12035-021-02353-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Effects of Euglena gracilis extract on Nrf2 signaling in LPS-stimulated cortical microglia. Microglia were cultured for 24 h in 10% serum-containing medium, which was replaced with serum-free medium before pre-treatment with EAE for 1 h followed by stimulation with 100 ng/mL LPS for 6 h. a Nrf2 and b HO-1 mRNA levels were quantified by real-time PCR. Data are presented as means ± SD (n = 3 in triplicate). *p < 0.05 and **p < 0.01 compared to control cells. ANOVA followed by Bonferroni’s post hoc test