Fig. 2. SETD2 is highly expressed during cortical development and required for neuronal migration.

a qPCR showed that the relative level of SETD2 mRNA was high in embryonic and early post-natal mouse cerebral cortex. All data were normalized to the P60 cerebral cortex and represented as the mean ± s.e.m. (n = 3 biological replicates). b Immunostaining showed a distribution of SETD2 in CP, IZ and VZ/SVZ of mouse somatosensory cortex at E14 and E16. Brain slices were stained for SETD2 (red), Tuj1 (green) and DAPI (blue) (n = 3 biological replicates). Scale bar: 25 μm. c SETD2 was localized in the NPCs of VZ/SVZ and the neurons of CP at E14 cerebral cortex. Dashed line indicated the lineament of cell and arrowheads indicated the SETD2 puncta in the cytoplasm. Brain slices were stained for SETD2 (red), Tuj1 (green) and DAPI (blue) (n = 3 biological replicates). Scale bar: 2.5 μm. d Representative images of coronal brain sections in somatosensory cortex at E16 showed the distribution of GFP⁺ cells (green) electroporated with GFP reporter as well as control shRNA (shNC), mSETD2 shRNA1 or mSETD2 shRNA2 at E14. Sections were stained for DAPI (blue). Scale bar: 25 μm. e Quantitative analysis of d showed that the percentage of GFP⁺ cells in IZ was significantly increased after SETD2 knockdown. All data were shown as the mean ± s.e.m. (n = 17, 10, 11, respectively). Data were analyzed using two-way ANOVA with Bonferroni’s post-hoc test. ***P < 0.001 versus shNC. f Representative images of shNC and shRNA2-expressing neurons (green) at E16 in the IZ. Brain slices were stained for DAPI (blue). Scale bar: 10 μm. g Quantitative analysis of f showed that the percentage of GFP⁺ neurons at the multipolar stage in IZ was significantly increased after SETD2 knockdown. All data were shown as the mean ± s.e.m. (n = 12, 16, respectively). Data were analyzed using two-way ANOVA with Bonferroni’s post-hoc test. ***P < 0.001 versus shNC. Source data are provided as a Source Data file.