Fig. 1. Global deletion of Tead1 in adult mice leads to rapid lethality due to acute-onset DCM.
a The breeding scheme to generate adult inducible global Tead1 KO mice (igKO) by crossing CAGGCre-ERTM+ mice with Tead1 flox (F) mice. b Survival curve of adult control (CAGGCre-ERTM-; Tead1F/F, N = 17 and CAGGCre-ERTM+; Tead1W/W, N = 18) and Tead1 igKO (CAGGCre-ERTM+; Tead1F/F, N = 12) mice following 5 daily intraperitoneal injections (IP) of tamoxifen. *p < 0.01. Kaplan–Meier analysis. c Control and Tead1 igKO mice were harvested at day 18 following the first tamoxifen injection. The organ weights then were normalized by the tibial length (TL). *p < 0.05 vs. control groups. d Representative echocardiographic M-mode images are shown from the control and Tead1 igKO mice prior (day 0) or at day 8 or 16 following the first tamoxifen injection. e Echocardiographic quantifications of control and Tead1 igKO mice. Shown are left ventricle (LV) ejection fraction (EF) and fractional shortening (FS) (upper panel), LV posterior wall thickness at end-diastole (LVPW;d) or at end-systole (LVPW;s) (middle panel), LV diastolic and systolic internal diameters (LVID;d and LVID;s, bottom panel). *p < 0.05 vs. control groups. f At 18 days following the first tamoxifen injection, hearts from control and Tead1 igKO mice were harvested for gross morphology (upper panel), sectioned for H&E staining (middle panel) and trichrome staining (bottom panel). Note thrombus accumulation in atria (arrows in upper panel), dramatic dilation of both LV and right ventricle (RV) chambers and thinning ventricular wall (arrows in middle panel) in Tead1 igKO mice.