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. 2021 Jun 21;7:166. doi: 10.1038/s41420-021-00509-w

Fig. 5. Depletion of NDUFA1 inhibits ESCC cell proliferation.

Fig. 5

A The short hairpin RNA (shNDUFA1) in the vector plko.1 was used to transduce KYSE150 and KYSE450 cells, and Western blotting detects the knockdown efficiency of selected shNDUFA1 in KYSE150 and KYSE450 cells. B Effect of shNDUFA1 in KYSE150 and KYSE450 cells cell growth. OD value was measured at 0, 24, 48, 72, 96 h by MTT assay. (*p < 0.05, **p < 0.01, ***p < 0.001). C Effect of shNDUFA1 in KYSE150 and KYSE450 cells anchorage-independent growth. Cells number was measured at 7 days. (*p < 0.05, **p < 0.01, ***p < 0.001). D Effect of shNDUFA1 in KYSE150 and KYSE450 cells colony formation. Cells number was measured at 12 days. (*p < 0.05, **p < 0.01, ***p < 0.001). E Effect of shNDUFA1 in KYSE150 and KYSE450 cells cell proliferation after cloperastine treatment. OD value was measured at 72 h by MTT assay. (*p < 0.05, **p < 0.01, ***p < 0.001).