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. 2021 Jul 5;4:836. doi: 10.1038/s42003-021-02357-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Water accessible surface (green spheres) in NorC’s vestibule starting from W139/K398 (black spheres), in the absence (left) and presence (right) of ICab. The outward-open conformation completely blocks accessibility from the cytosolic side of NorC. b Microscale thermophoresis profiles for NorC and tetraphenylphosphonium (TPP⁺) in the presence and absence of ICab. K_d = 4 ± 0.8 μM for NorC and TPP⁺. n = 4 for NorC-TPP⁺ titration for independent replicates. The value provided is a concurrent representative from one. c ITC traces of ICab titrated against NorC WT in the presence and absence of TPP⁺ (offset corrected differential power, top panel; baseline subtracted binding isotherm with integrated peaks normalized against moles of injectant, bottom panel) concurs with mode “B” of TPP⁺ and ICab binding. n = 2 for independent replicates. Source data is provided in the source data file.