Skip to main content
. 2021 Jun 17;7:165. doi: 10.1038/s41420-021-00534-9

Fig. 2. The effects of the absence of miR-146a on immune rejection after heart transplantation.

Fig. 2

A Kaplan-Meier analysis of the allograft survival time (log-rank test), n = 6. B Assessment of PR scoring and CAV grading of allografts using H&E staining according to the ISHLT guidelines, n = 6. C Histological analyses of the harvested allografts stained with H&E (×400; scale bars = 50 μm; n = 6) and D Masson’s trichrome (×200; scale bars = 50 μm; n = 3) were performed on day 5 after transplantation in the WT, WT+RAPA, CKO, and CKO+RAPA groups. E The infiltration of CD4+ and CD8+ T cells in the allografts of the WT, WT+RAPA, CKO, and CKO+RAPA groups on day 5 were detected using immunofluorescence staining (×400; scale bars = 50 μm), n = 3. F The infiltration of Tregs (Foxp3+) in the allografts of the WT, WT+RAPA, CKO, and CKO+RAPA groups on day 5 were detected using immunohistochemistry (×400; scale bars = 50 μm), n = 3. Data are presented as means ± SEM. *p < 0.05 and **p < 0.01. CKO indicates miR-146a conditional knockout mice. WT wild type, RAPA rapamycin.