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. 2021 Jun 17;7:165. doi: 10.1038/s41420-021-00534-9

Fig. 3. The effects of the absence of miR-146a on the function of allografts and immune response in recipients after heart transplantation.

Fig. 3

A The expression of HMGBI in the allografts was detected using immunohistochemistry (×400; scale bars = 50 μm) on day 5 in the WT, WT+RAPA, CKO, and CKO+RAPA groups, n = 3. B Serum levels of Troponin T were detected in recipient mice from all groups on day 5 using an ELISA. n = 3. C The flow cytometry analysis of the proportions and numbers of Tregs (CD4+ Foxp3+) in the spleens of recipients was performed on day 5 in all groups, n = 3. D The flow cytometry analysis of the proportions and numbers of M1 (CD16/32+ CD68+) and M2 (CD206+ CD68+) macrophages in the spleens of recipients was performed on day 5 in all groups, n = 3. E–F Serum levels of IL-10 and TGF-β1 were detected in recipients from all groups on day 5 using ELISAs, n = 3. Data are presented as means ± SEM. *p < 0.05 and **p < 0.01. CKO indicates miR-146a conditional knockout mice. WT wild type, RAPA rapamycin.