Fig. 5. The effects of the absence of miR-146a in Tregs on macrophage polarization.

A Monocytes from BALB/c spleens were co-cultured with or without WT-Tregs or CKO-Tregs and different stimuli for 72 h, and a flow cytometry analysis of the proportions of M1 (CD16/32⁺ CD68⁺) and M2 (CD206⁺ CD68⁺) macrophages was performed, n = 3. B–C The levels of TNF-α, IL-1β, and IL-10 in the supernatant of the co-cultures of monocytes and Tregs were detected using ELISAs. n = 3. Data are presented as means ± SEM. *p < 0.05 and **p < 0.01. St indicates the corresponding stimuli. CKO indicates miR-146a conditional knockout mice. WT-Treg and CKO-Treg indicate Tregs isolated from WT and miR-146a CKO mouse spleens, respectively. WT wild type, Treg regulatory T cell.