Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 26;7:157. doi: 10.1038/s41420-021-00552-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A Developmental expression profiles of miR-133a and miR-499. The expression changes were calculated by comparing with E14.5 data. B Developmental global m6A modifcation levels, as determined by ELISA analysis. ns, no significant as compared to P1d. C, D Heatmap showing the developmental changes of miR-133a and miR-499 target protein expression from the published proteomics database. The ventricular tissues were collected from the postnatal day 1 (P1) to P23 of the C57BL/6 mouse. E The percentage of the down- and upregulated proteins (Ratio of P23d to P1d) by miR-133a and miR-499. F The proportions of down- and upregulated genes targeted by two miRs with or without m6A modification. G Quantitative western blot analysis showed that the decreased CDC42 and TEAD1, two miR-133a target proteins in the developing ventricle from P1d to postnatal 5 months (P5m). Error bars, SD (n = 4–5/stage), *P < 0.05 as compared to P1d. H Enrichment of m6A modification at Cdc42 and Tead1 was analyzed by m6A-RNA immunoprecipitated-qPCR method. Data are mean ± SD (n = 4–6 per stage). *P < 0.05; ***P < 0.001 was calculated using the one-way ANOVA followed by Tukey’s test.