Skip to main content
. 2021 Feb 10;28(7):2112–2125. doi: 10.1038/s41418-021-00739-6

Fig. 6. p53 is necessary for FBXL6-mediated malignant phenotypes.

Fig. 6

a Western blot analysis of lysates from RKO, HCT116, and HCT116 p53−/− cells stably expressing the indicated vectors. β-actin was used as a loading control. b Focus formation by RKO, HCT116 and HCT116 p53−/− cells stably expressing the indicated vectors. Colony numbers are shown in the bar graphs. c MTT assays were performed in the above cells from (b). d, e Xenograft growth in nude mice. Indicated CRC cells stably expressing the indicated vectors were subcutaneously injected into nude mice (n = 5). Tumor volumes were measured every 3 days. f The ratio of each cell phase in RKO, HCT116 and HCT116 p53−/− cells stably expressing indicated vectors. RKO, HCT116 and HCT116 p53−/− cells were stained with PI and analyzed by flow cytometry. g The ratio of apoptosis in RKO, HCT116 and HCT116 p53−/− cells stably expressing indicated vectors. The above cells were stained with Annexin V-FITC and PI and then analyzed by flow cytometry. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.