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. 2021 Jun 14;28(7):2145–2159. doi: 10.1038/s41418-021-00743-w

Fig. 3. AR-circRNA-ARC1 regulates PCa vs. BCa cell invasion through miR-125b-2-3p and/or miR-4736.

Fig. 3

A The miRNAs can repress cell invasion induced by Enz in PCa C4-2 cells. Invasion assay was performed in C4-2 cells transduced with the indicated lentiviral constructs with Enz treatment (10 μM, 48 h). The miRNAs can repress cell invasion induced by overexpression of AR in BCa UMUC3 (B) and TCC-5637 (C) cells. Invasion assays were performed in BCa cells transduced with the indicated lentiviral constructs. For A–C top panels, representative microscopic photographs of invaded cells (original magnification, ×100) were shown, with lower panels quantitation of images (cell numbers were counted in six randomly chosen microscopic fields per membrane). Scale bar: 20 µm. D The circRNA-ARC1 (ARC1) can interact with miRNA-125b-2-3p and miR-4736 in PCa and BCa cells. ARC1 was pulled-down on the streptavidin beads by biotinylated oligonucleotide complimentary to the fusion junction of the circRNA-ARC1. The miRNAs associated with the ARC1 were determined through quantitative PCR of the respective miRNAs in PCa CWR22Rv1 and BCa TCC-SUP cells with or without overexpression of ARC1. E miR-125b-2-3p can suppress ARC1-induced cell invasion in PCa C4-2 (upper panels) and BCa UMUC3 (lower panels) cells. F miRNA-125b-2-3p inhibitor can rescue cell invasion repressed by ARC1-knocked down in PCa CWR22Rv1 (upper panels) and BCa T24 (lower panels) cells. For E, F left panels are representative microscopic photographs of invaded cells and right panels the quantitation of cell invasion. Scale bar: 20 µm. Data are presented as mean ± SEM. *P < 0.05.