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. 2021 Jun 14;28(7):2145–2159. doi: 10.1038/s41418-021-00743-w

Fig. 5. In vivo mice studies confirmed the role of Enz and ARC1 in PCa metastasis.

Fig. 5

A IVIS imaging was used to determine the metastasis in each group of mice as indicated. The images were obtained at different times with the same exposure and digitally arranged. B Representative images of pelvic lymph node, diaphragm, mesentery metastasis (upper panesl) with IHC staining in lower panels. C Tabulation of mice with metastasis. Metastatic site tabulations were listed. Chi-square test was used to analyze the results (upper panel), Enz significantly promoted CWR22Rv1 PCa metastasis (15 mice in Enz treated group (n = 32) vs. 6 mice in vehicle-treated group (n = 32), P = 0.015) and Enz failed to promote tumor metastasis when circRNA-ARC1 was knocked down (lower panel). The Pearson’s chi-square test shows significant differences among all four groups (P value = 0.0349). D H&E staining confirmed the tumor tissue and representative images of IHC staining for AR and MMP-9 with quantitations (lower panels) on the right as mean ± SEM. *P < 0.05 and ns = no significant difference.