Skip to main content
. 2021 Jul 5;12:4132. doi: 10.1038/s41467-021-23889-0

Fig. 5. Equalizer-L has superior gene dosage compensation than an alternative circuit that combines post-transcriptional NF and IFF motifs.

Fig. 5

a–d Circuit schematics. mScarlet-I (RFP) and mCitrine (YFP) are reporters of circuit output and gene dosage, respectively. CMV (c) and OLP (d) do not have dosage compensation circuitry and are used as controls for Equalizer-L (a) and HYB (b), respectively. e Representative circuit output histograms. For all experiments (e–h), Equalizer-L was induced with 1 ng/mL of doxycycline. f HYB produces high cell-to-cell circuit output variability. The gene-dosage values were normalized to those obtained when transfecting 1 ng of plasmid. The circles represent independent transfections. n = 36 per circuit (6 per dose and 6 doses per circuit). The dashed lines are trend lines (linear for Equalizer-L and CMV; exponential for HYB and OLP). The inset shows the trend lines for the entire range of gene-dosage reporter levels. See Supplementary Statistics for statistical comparisons. Equalizer-L (g) showed superior gene dosage compensation than HYB (h) at the population level. The gene-dosage and circuit output values were normalized to those obtained when transfecting 1 ng of plasmid. Dashed lines indicate trend lines (hyperbolic for CMV and linear otherwise). Sample sizes are as in (f). p < 0.0001 for the two-sided Welch’s t-test comparing the trendline slopes of Equalizer-L and HYB. Source data are provided as a Source Data file.