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. 2021 Jun 29;7:150. doi: 10.1038/s41420-021-00548-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Diagram of the experimental procedure: rat pups received intracerebroventricular (icv) injection of recombinant adenovirus IRS-1 (Ad-IRS-1) or control adenovirus (Ad-control) on postnatal (P) day 5, had hypoxic-ischemic (HI) brain injury on P7, and scarified at 24 h after HI for outcome measurements. B Expression of HA-tagged IRS-1 mediated by Ad-IRS-1 on NeuN (+) neurons and RECA(+) endothelium cells in the cerebral cortex of P7 rat pups. N = 4, Scale bar: 50 μm. C The Ad-IRS-1-treated rats showed upregulate the expression of IRS-1 in the brain, and reduced cleaved-PARP, and –capsease 3 after HI compared to the Ad-control treated rats. N = 5. D The expression of cleaved caspases-3 in NeuN (+) neurons was obvious in the Ad-control-infected rats at 24 h after HI compared to the Ad-IRS-1-infectedrats. N = 4, Scale bar: 50 μm. E, F Representative images illustrate immunoglobulin (IgG) and albumin extravasation in the cerebral cortex at 24 h after HI between the Ad-IRS-1-infected and Ad-control-infected rats, and the relative integrated optic density (IOD) of IgG (N = 4, P = 0.001, t = 6.305) and albumin (N = 4, P < 0.001, t = 14.644) signals was compared. Scale bar: 125 um. Independent t-test was used for statistical analysis. All data are presented as mean ± SD. ** P < 0.01. G The Ad-IRS-1 rats had more perivascular fibrin deposits than the Ad-control rats at 24 h after HI. N = 4, Scale bar: 50 μm.