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. 2021 Jun 29;7:150. doi: 10.1038/s41420-021-00548-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Diagram of the experimental procedure in the endothelial-specific IRS-1 transgenic rats (eTg/0) and wild-type littermates (eWT) rats: HI on P7, and outcome assessments on P8 and P21. The transgene contains endothelial-specific promoter (Tie2), full-length IRS-1 tagged with 3x Flag and IRES-directed tdTomato. B IRS-1/tdTomato was expressed specifically on RECA (+) endothelium cells, and not on NeuN(+) neurons, GFAP(+) astrocytes, or Iba-1(+) microglia in the cerebral cortex of P7 rat pups. Scale bar: 50 μm. C The eTg/0 rats had increased levels of cleaved-PARP, and –capsease 3 after HI compared to the eWT rats. N = 4. D Increased cleaved caspase 3 (+) and NeuN (+) apoptotic neurons were noted in the eTg/0 rats compared to that in the eWT rats at 24 h after HI. Scale bar: 50 μm. N = 4. E Representative images illustrate albumin extravasation in the cerebral cortex at 24 h after HI between eTg/0 and eWT rats, and the relative integrated optic density (IOD) of albumin signal was quantified. Scale bar: 125 μm. Independent t-test was used for statistical analysis (N = 6, P = 0.001, t = −5.040). The data are presented as mean ± SD. * P < 0.05. F The extravascular fibrin is more in the eTg/0 rats compared to that in the eWT rats at 24 h after HI. Scale bar: 50 μm. Inset scale bar: 50um. N = 4. G Immunoblotting analysis of tight junction proteins in the brain of eTg/0 rats. N = 4. H The brain infarct volume was compared between eTg/0 and eWT rats. Independent t-test was used for statistical analysis (N = 12, P = 0.002, t = −3.638). The data are presented as mean ± SD. ** P < 0.01.