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. 2021 Jun 29;7:150. doi: 10.1038/s41420-021-00548-3

Fig. 6. mTOR inhibition reduced HI brain damages in the endothelial-specific IRS-1 transgenic rats.

Fig. 6

A Compared to vehicle, rapamycin treatment greatly decreased the cleaved-PARP and -caspase 3 levels after HI in the eWT and eTg/0 pups. N = 4. B The cleaved caspase 3 (+) and NeuN (+) apoptotic neurons were reduced in the rapamycin (Rapa)-treated eTg/0 rats than that in the vehicle (Veh)- treated eTg/0 rats at 24 h after HI. Scale bar: 50 μm. N = 4. C, D The extravasation of IgG and albumin in the cerebral cortex at 24 h after HI reduced in the rapamycin-treated eTg/0 pups compared to that of vehicle-treated eTg/0 pups. N = 6, Scale bar: 125um. One-Way ANOVA (Scheffe’s post-hoc test) was used for statistical analysis (C: F value = 8.617; D: F value = 16.445). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01. E Expression of tight junction proteins, ZO-1, occludin and claudin-5, were relatively preserved by treatment of rapamycin at 24 h after HI compared to vehicle in the eTg/0 and the eWT rats. N = 4. F The brain infarct volume on P21 (14 days after HI) was quantified. The brain infarct volume was significantly reduced after treatment of rapamycin in the eTg/0 (N = 12) or eWT rats (N = 10). One-Way ANOVA (Scheffe’s post-hoc test) was used for statistical analysis (F value = 11.968). * P < 0.05.