Fig. 1.

Schematic representation of the strategy for plasmid construction and the production of recombinant ZIKV and ZIKV-GFP. A Illustration of the ZIKV genome and constructs used in this study. In the pZIKVrepdCME construct, the GFP- and FMDV-2A-coding sequences were inserted downstream of sequences encoding the first 20 amino acids of the C gene and upstream of sequences encoding the last 22 amino acids of the E gene. The 3′ untranslated region was flanked with HDVr to ensure the authenticity of the 3′ terminus of the transcribed RNA. B Schematic diagrams for the production of recombinant ZIKV and ZIKV-GFP marker viruses. DNA fragments containing the CMV promoter and the coding sequences of structural proteins were amplified by PCR. The purified PCR products and enzyme-linearized ZIKV replicon plasmid DNA were pooled and transfected into HEK-293T cells to generate infectious ZIKVs.