Fig. 3.

High-throughput screening for inhibitors of ZIKV infection from a selected plant-sourced compound library. A High-throughput screening (HTS) assay timeline. BHK-DR cells were seeded in CellCarrier-96 microplates. After incubation for 16 h (usually overnight), cells were treated with compounds. One hour later, cells were infected with ZIKV-GFP for 48 h. The final concentration of compounds was 10 μmol/L. The final concentration of vehicle (DMSO) was 1%. Cell nuclei were stained with Hoechst for 20 min. The plates were scanned using a high-content-screening microplate imaging reader. B High-throughput screening of a library of 974 selected plant-sourced compounds. Each circle represents the percentage inhibition achieved with each compound at a concentration of 10 μmol/L. The circles within the blue box represent an inhibition > 80%. In total, 103 compounds causing a virus infection inhibition rate of > 80% were selected for further analysis of relative cell viability. C Out of 103 compounds from the primary selection, 31 compounds represented by red hollow circles within the blue-dotted square passed the criterion of relative cell viability > 80% at a concentration of 10 μmol/L. These compounds were selected for a confirmatory screen. D IC₅₀ and CC₅₀ of the top four selected compounds.