Fig. 4.

Validation of the antiviral effects of the hit compounds. A Top: The chemical structures of the hit compounds homoharringtonine (HHT), dihydroartemisinin (DHA), digitonin (DGT) and bruceine D (BD). Bottom: Dose–response curves showing the effect of compound treatment on virus infection inhibition (red) and cell viability (blue) in BHK-DR cells infected with ZIKV-GFP. Values represent the means ± SD from two independent experiments performed in triplicate. The data are normalized to those of DMSO-treated cells. B Representative fluorescence images of BHK-DR cells treated with varying concentrations of hit compounds. BHK-DR cells were treated with the indicated compounds at the indicated final concentrations and were infected after 1 h with reporter virus at an MOI of 0.1. Cells treated with DMSO at a final concentration of 1% were used as a vehicle control. At 48 hpi, cell nuclei were stained with Hoechst and cell plates were scanned using a high-content-screening microplate imaging reader.