Fig. 6.
The K492R/K532R mutation does not affect N-P interaction or P oligomerization formation as well as the formation of inclusion bodies. A 293T cells were transfected with N-Myc and HA-P/PK492R/K532R, mock was transfected with individual HA-P/PK492R/K532R. At 36 h post-transfection, cells were lysed and N-Myc was immunoprecipitated with anti-Myc agarose. The precipitated proteins and lysates were further analyzed by SDS-PAGE and immunoblotting with anti-HA polyclonal antibodies and anti-Myc polyclonal antibodies. B As similar with (A), 293T cells were transfected with Myc-P and HA-P/PK492R/K532R, mock was transfected with individual HA-P/PK492R/K532R. At 36 h post-transfection, cells were lysed and Myc-P was immunoprecipitated with anti-Myc agarose. The precipitated proteins and lysates were further analyzed by SDS-PAGE and immunoblotting with anti-HA polyclonal antibodies and anti-Myc polyclonal antibodies. C HeLa cells were transfected with plasmids encoding HA-P/PK492R/K532R or N-Myc alone or together. At 24 h posttransfection, cells were fixed and stained with antibodies against HA and Myc. The subcellular locations were visualized via confocal microscopy as described in Materials and Methods. DAPI was used for the nuclear staining. Scale bars, 10 μm.