Figure 2.

NRP1 and NRP2 RNA and protein expression in HB cell lines. NRP1 mRNA expression was significantly higher in 4/6 HB cell lines investigated compared to adult primary hepatocyte control (A). Similarly, NRP1 protein expression was elevated in same four cell lines (B) compared to pediatric primary hepatocytes. Upregulated NRP2 expression was observed in 5/6 HB cell lines both at mRNA (C) and protein level (D). ^§Adjusted p-value < 0.05. aPH, adult primary hepatocytes; pPH, pediatric primary hepatocytes. Band intensity is normalized to total protein expression of each lane. Normalization factor (NF) describing the amount of total protein in lane in relation to other lanes is given beneath the bands (B,D).