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Three recently emerged enzymatic oligo synthesis methods. (A) Two-step extension synthesis of DNA oligo using TdT and dNTPs with reversible blocking groups. (B) Two-step extension synthesis of DNA oligo using TdT–dNTP conjugates for reversible termination of oligo elongation. (C) Template-dependent polymerase mediated oligo synthesis by transient hybridization and chemically blocked substrates.
