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. 2021 Jun 22;9:672847. doi: 10.3389/fcell.2021.672847

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Copyright © 2021 Li, Liao, Luo, Song, Wang, Feng, Ou, Wu, Zhang, Gao, Yin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AIM2 expression in human intervertebral disc tissues. (A) AIM2 mRNA level measured by qRT-PCR in non-degenerative NP tissues (NDC) and degenerative NP tissues (IDD), n = 15. (B) Correlation analysis between the mean AIM2 mRNA level and Pfirrmann grades of NDC and IDD tissues. (C) Represent western blot image and quantification of AIM2 protein expression in human NDC and IDD tissues. GAPDH was used as an internal control. (D) HE staining and immunochemistry staining of γ-H2AX and AIM2 in human NDC and IDD tissues. (E) Quantification rate of AIM2 and γ-H2AX positive cells. Data were presented as the means ± SD, n = 3. **P < 0.01, ***P < 0.001 vs. NDC group.