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. 2021 Jun 22;9:672847. doi: 10.3389/fcell.2021.672847

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Copyright © 2021 Li, Liao, Luo, Song, Wang, Feng, Ou, Wu, Zhang, Gao, Yin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GRASP55 regulates AIM2 unconventional secretion and autophagy activity. (A) Immunoprecipitation for AIM2 was conducted to detect the integration of GRASP55 or GRASP65. (B,C) Confocal images of AIM2 (green) and GRASP55 (red) (B) and overlap coefficient (C) in H₂O₂-treated NP cells. (D) Knockdown of GRASP55 in NP cells and corresponding western blot images of GRASP55 and AIM2. (E) Quantification of AIM2 and GRASP55 in H₂O₂-treated NP cells with si-GRASP55 or si-scr. (F) Extracellular levels of AIM2 measured by ELISA analysis. (G,H) Protein levels (G) and quantification (H) of GRASP55, LC3 and p62 in H₂O₂-treated NP cells with si-GRASP55 or si-scr. Data were presented as the means ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group. (I) TEM images of NP cells showed the autophagosomes (red arrows) and other irregular intracellular vesicles (blue arrows).