Fig. 3.

Dose response for compounds 1 and 2 in PEDV-infected Vero cells: A Immunofluorescence assay of compounds 1 and 2 against PEDV in Vero cells. PEDV-infected cells were used as a control group. Serial dilutions of the test compounds from 3.125 µmol/L to 25 µM were assessed independently. Nuclei were stained with DAPI (blue fluorescence), and PEDV infection was detected using a monoclonal antibody followed by FITC-tagged goat anti-rabbit IgG (green fluorescence). B Western blot analysis of the effects of compounds 1 and 2 on the production of CoV N protein in PEDV-infected cells. GAPDH was used as an internal control.