Skip to main content
. 2021 Jun 22;12:662020. doi: 10.3389/fphar.2021.662020

FIGURE 2.

FIGURE 2

The damage biomarker KIM-1 is overexpressed in injured tubules of old mice. Folic Acid (FA; 125 mg/kg) was injected in 3-months-old (Young) and 1-year-old (Old) C57BL/6 mice and kidneys were studied after 48 h. (A) Total mRNA was isolated from frozen sections of whole kidneys and qRT-PCR was performed to determine gene expression levels of Havcr-1. (B) Representative microphotographs of KIM-1 expression levels evaluated by immunohistochemistry and (C) its quantification of stained area per total area. Scale bars = 100 μm. Data are shown as n-fold and expressed as mean ± SD of n = 6–9 animals per group. *p < 0.05 vs. control young mice, # p < 0.05 vs. FA-injected young mice, & p < 0.05 vs. control old mice. The non-parametric Kruskal-Wallis statistical test was performed.