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. 2021 Jun 22;12:662020. doi: 10.3389/fphar.2021.662020

FIGURE 3.

FIGURE 3

Characterization of inflammatory infiltrate in the acute phase of folic acid (FA) nephropathy in young and old mice. Folic Acid (FA; 125 mg/kg) was injected in 3-months-old (Young) and 1-year-old (Old) C57BL/6 mice and kidneys were studied after 48 h. Inflammatory cell infiltration was evaluated using antibodies against myeloperoxidase (neutrophils), F4/80 (monocytes/macrophages/dendritic cells) and CD3 (T lymphocytes). (A) Representative micrographs from each group. Scale bars = 100 μm. (B–D) Quantification of MPO (B), F4/80 (C), and CD3 (D) positive cells. Data are shown as n-fold and expressed as mean ± SD of n = 6–9 animals per group. *p < 0.05 vs. control young mice, # p < 0.05 vs. FA-injected young mice, & p < 0.05 vs. control old mice. The parametric one-way ANOVA statistical test was performed.