FIGURE 6.

Upregulation of the necroptosis pathway in the acute phase of folic acid (FA) nephropathy in old and young mice. Folic Acid (FA; 125 mg/kg) was injected in 3-months-old (Young) and 1-year-old (Old) C57BL/6 mice and kidneys were studied after 48 h. The renal gene expression levels of Ripk3 (A) and mlkl (B) were evaluated by qRT-PCR. (C–F) MLKL protein and active caspase-3 (represented by cleaved caspase-3) were determined by Western blot. α-tubulin was used as loading control. (C, E) Representative blots and (D, F) their quantification. Data are shown as n-fold and expressed as mean ± SD of n = 5–9 animals per group. *p < 0.05 vs. control young mice, ^# p < 0.05 vs. FA-injected young mice, ^& p < 0.05 vs. control old mice. The parametric one-way ANOVA statistical test was performed.