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. 2021 Jun 22;12:662020. doi: 10.3389/fphar.2021.662020

FIGURE 8.

FIGURE 8

Increased expression of senescence-associated secretory phenotype components (SASP) and anti-apoptotic proteins in old injured kidneys. Folic Acid (FA; 125 mg/kg) was injected in 3-months-old (Young) and 1-year-old (Old) C57BL/6 mice and kidneys were studied after 48 h. Gene expression levels of Il6 (A), Tgfβ (C), Serpine1 (D), and Ctgf (E) were determined by qRT-PCR. (B) Total protein of renal extracts of IL-6 were evaluated by ELISA. (F) Gene expression levels of Bcl2l1 were evaluated by qRT-PCR. (G, H) BCL-xL protein was evalated by immunohistochemistry. (G) Representative microphotographs of BCL-xL and (H) its quantification of stained area per total area. Data are shown as n-fold and expressed as mean ± SD of n = 6–9 animals per group. *p < 0.05 vs. control young mice, # p < 0.05 vs. FA-injected young mice, & p < 0.05 vs. control old mice. The parametric one-way ANOVA statistical test was performed, except for the BCL-xL quantification, in which a non-parametric Kruskal-Wallis statistical test was conducted.