Figure 2 .

Embryonic transient ALK inactivation induced anxiety-like behaviors and impaired recognition memory in adult mice. (A) Movement heatmaps of the mice embryonically exposed to ceritinib and vehicle in the open field apparatus. (B) Total distance traveled, (C) the mean travel speed, (D) entries to the center, and (E) total time spent in the center of the open field. (F) Movement heatmaps of the mice in the elevated plus maze test. (G) Time spent in the open arms and (H) the ratio of time spent in open arms to total time spent in both open and closed arms. (I) Number of marbles buried by vehicle and ceritinib mice. (J) Immobility time in the tail suspension test. (K) Movement heatmaps of the mice embryonically exposed to vehicle or ceritinib in novel object test. (L) Recognition index showing impaired preference to the novel object in the ceritinib group. (M) Vehicle but not ceritinib mice showed preference to the novel over the familiar object. All data are presented as mean ± SEM. n = 10 mice per group. Statistical significance was determined by unpaired Student’s t-test (B–E, G–H, I–J, L); paired Student’s t-test (M). ^*P < 0.05, ^**P < 0.01.