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. 2021 Mar 19;31(8):3804–3819. doi: 10.1093/cercor/bhab050

Figure 5 .

Figure 5

Muscarinic receptors–mediated cholinergic activation mediates enhancement of AMPAR mEPSCs frequency at hippocampal CA1 excitatory synapses. (A) Representative traces of AMPAR mEPSCs. (B) Cumulative distributions of event amplitudes and mean event amplitudes (inset) display no significant difference in mEPSCs amplitude. (C) Cumulative distributions of interevent intervals and mean event frequencies (inset) demonstrate that enhancement of AMPAR mEPSCs frequency, which is mediated by MMP-9 inhibition and Cch, is impaired by cholinergic muscarinic receptors antagonist (Scop). Data shown as mean ± SEM (numbers of neurons/independent cultures). Statistical comparisons were performed using Kolmogorov-Smirnov test on cumulative distributions and nested one-way ANOVA followed by Sidak’s test for bar graph inserts (mEPSCs frequency: F3,16 = 10.26, Inh I + Cch vs. Ctrl P = 0.0009, Scop + Inh I + Cch vs. Inh I + Cch P = 0.01). Numbers inside each bar represent the numbers of neurons/slices/cultures. Statistical analysis was based on the number of slices (P value: *P < 0.05, ***P < 0.001).