Figure 2.

8-oxo-guanine (8-oxoG) Staining in glomeruli of patients with focal segmental glomerulosclerosis (FSGS) indicates increased oxidative stress and correlates with ET_AR expression. (a) Representative examples of 8-oxoG staining in (left) a transplant control kidney sample and (right) a biopsy sample of a patient with FSGS. (b) Quantification of glomerular 8-oxoG as measured by ImageJ. Graph shows the summary data of the mean 8-oxoG−positive area per patient. Although the median 8-oxoG expression was not different in patients with FSGS compared to controls, there was a subset of FSGS patients with increased 8-oxoG levels (as indicated by the higher spread in the upper quadrants). (c) In patients with FSGS, glomeruli with ET_AR-positive endothelium showed more 8-oxoG−positive staining. (d) Occasionally, we observed colocalization of 8-oxoG−positive cells within areas of nephrin loss (arrowheads). Bars = 50 μm.