Figure 1.

HSP60-knockdown suppresses the proliferation of colorectal cancer cells in vitro and in vivo. (A) HSP60 was highly expressed in colorectal cancer tissues (red) compared with in paired normal tissues (gray) from patients with colorectal cancer. The log₂-transformed values of HSP60 gene expression of the COAD dataset in GEPIA were used to compare the difference between cancer and normal samples. Significance was calculated using the Wilcoxon rank-sum test. (B) HSP60 was knocked down in HSP60-KD1 and -KD2 cells. (C) Cell Counting Kit-8 assay indicated that cell proliferation was significantly decreased in HSP60-KD cells than in control cells. (D and E) HSP60-knockdown inhibited tumor growth in nude mice. The volumes of xenograft tumors of HCT HSP60-KD and control cells were monitored for 20 days after injection. Data were analyzed using ordinary two-way ANOVA followed by Bonferroni's test as multiple comparisons test. (F) Florescent intensities of tumors on the 20th day after injection indicated the relative size of xenograft tumors generated from HSP60-KD and control cells in nude mice. Data were analyzed using ordinary one-way ANOVA followed by Benjamini-Hochberg method as multiple comparisons test. *P<0.05; ***P<0.001. HSP60, heat shock protein 60; KD, knockdown; T, tumor; N, normal; TPM, transcripts per million; COAD, colon adenocarcinoma; GEPIA, Gene Expression Profiling Interactive Analysis.