Figure 5.

HSP60-knockdown in HCT116 cells activates the AMPK signaling pathway and inhibits mTOR and protein synthesis. (A) Western blotting indicated that phosphorylation of AMPK (T172) in HSP60-KD HCT116 cells was increased compared with in control cells. (B) Western blotting indicated that the mTOR signaling pathway was inhibited in HSP60-KD cells compared with in control cells. (C) Diagram illustrating the effects of HSP60-knockdown on the adenine/AMPK/mTOR signaling pathway and proliferation of HCT116 cells. HSP60, heat shock protein 60; KD, knockdown; p, phosphorylated; 4EBP1, eukaryotic translation initiation factor 4E binding protein 1; p70S6K, p70S6 kinase.